Abstract
This work sought to apply the four-component simplex lattice experimental design to determine the chemical interaction between Terminalia chebula Retz. var. chebula, Terminalia arjuna Wight and Arn., Terminalia bellirica (Gaertn.) Roxb., and Phyllanthus emblica L., which are found in the traditional Chatuphalathika remedy. The four phenolic compounds, gallic acid, corilagin, chebulagic acid, and chebulinic acid, were quantified using validated high-performance liquid chromatography. A response surface analysis was also conducted to describe the level of interaction among the four plant ingredients. The results showed that a low content of P. emblica in the herbal remedy exhibited a positive effect on the extraction yield and the phenolic compounds content, except gallic acid. According to the combination index value, the low content of P. emblica in the herbal remedy revealed a positive effect on the interaction of extraction yield and the phenolic compounds content. The maximum interaction was found at an equal weight ratio of T. chebula, T. arjuna, and T. bellirica, with no P. emblica. The formula which boasts the equal weight ratio of three Terminalia plants exhibited a 167% increase in phenolic compounds as compared with the effects of any of the plants individually. Data from this work could be used to describe the synergy of plant ingredients contained in the Chatuphalathika remedy based on the chemical analysis aspect.
… The four phenolic compound standard markers—
chebulagic acid (purity
99.62%, HPLC),
chebulinic acid (purity 98.42%, HPLC),
corilagin (purity
99.67%, HPLC), and
gallic acid (purity 99.88%, HPLC), were purchased from Chengdu
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