Abstract
Ethnopharmacological relevance
Compound Danshen tablet, an herbal preparation consisting of salviae miltiorrhizae, notoginseng and borneolum, is extensively employed clinically to treat angina pectoris, coronary arteriosclerosis and significantly improve microcirculation.
Aim of the study
To reveal the potential underlying cardioprotective mechanism(s) in isoproterenol-induced myocardial injury in high-fat-diet fed mice.
Materials and methods
Cardiac transcriptomics was analyzed by Illumina mRNA-Seq sequencing. The restored cardiovascular diseases (CVD)-related genes by Compound Danshen tablet were validated by quantitative real time polymerase chain reaction (qRT-PCR). Furthermore, Cardiac metabolomics were also performed using gas chromatography-mass spectrometry.
Results
From the transcriptomics study, we found the levels of 24 up-regulated and 44 down-regulated genes in the control compared to model groups. Among them, seven gene levels were restored by treatment of Compound Danshen tablet. Four CVD-related genes at the mRNA level (Sprr1a, Ppp1r3c, Bmp10 and Hspa1b) were validated successfully by qRT-PCR. From the metabolomics study, 37 differentially expressed metabolites were identified between the control and model groups. Among them, 21 metabolites were restored by treatment of Compound Danshen tablet. These altered metabolites are involved in glucose metabolism, fatty acid metabolism and amino acid metabolism.
Conclusion
These genes and metabolites might provide clues for further molecular mechanistic study of Compound Danshen tablet.
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ginsenoside Re,
ginsenoside Rb1,
notoginsenoside R2,
ginsenoside Rg2,
ginsenoside Rh1,
ginsenoside Rd,
notoginsenoside Fe,
ginsenoside Rc,
ginsenoside Rb3,
notoginsenoside Fc and
notoginsenoside Fa were from
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