Abstract Theobjectiveof this studyis todevelopthequalitativeandquantitativeanalytical methodofginger rhizome by monitoringthepungent ingredients includinggingerols andshogaolsbyusing UltraPerformance Liquid Chromatographic (UPLC) technique.For qualitative analysis of ginger, 300 mg of powdered ginger was macerated in 20.0 mlof methanol for24h.Supernatant solution waspipettedandfilteredthroughthenylonsyringefilter.3µlof sample solution was injectedintothe chromatographic system consistingof AcquityTM BEH C182.1×50 mm,1.7 µm column and 40-65% of acetonitrile solution in water as mobile phase. Gradient elution was performed.Flow rate was set at0.6 ml/min. Chamber temperatureof sample chamber andcolumn were35and40°C, respectively. Detection wasperformedat the UV wavelengthof226nm.6active compounds including6-gingerol,8-gingerol, 10-gingerol, 6-shogaol, 8-shogaol and 10-shogaol were detected at the retention times at 1.71, 2.39, 2.77, 2.45, 2.91and3.65 min, respectively. This system couldbe alsousedfordeterminationof6-gingerol content. Thevalidation result of the developed method exhibited the linearity of calibration curve of 6-gingerol was in the range of 0.0474-0.2758 mg/ml with coefficient of determination (R2 ) 0.9998. Accuracy and precision were in the range of 100.68-101.19% and 0.43-0.51%, respectively. Limit of detection and limit of quantitation were 0.0038 and 0.0127 mg/ml, respectively. When 8 samples of ginger were analysed, the content of 6-gingerol was obtained in the range of 0.50-1.49% w/w
… 2. สารมาตรฐาน
6-gingerol ความบริสุทธิ์ ≥ 98% (Chengdu
Biopurify Phytochemicals, China) 8-gingerol ความบริสุทธิ์ ≥ 95% (Sigma- Aldrich, Switzerland) … USA)
6-shogaol ความบริสุทธิ์ ≥ 98% (Chengdu
Biopurify Phytochemicals, China)
8-shogaol ความบริสุทธิ์ ≥ 98% (Chengdu …
