Abstract
Background
During the development of Multiple Sclerosis (MS) there is a marked activation of microglia and astrocyte, leading to progressive inflammation and degeneration ofmyelin sheath which results in axonal loss and neuron damage.
Purpose
In this study, we have explored the action of Asiaticoside A against the activated microglia and astrocytes.
Methods
Primary microglia and astrocyte cultures were used for the study and the activity were evaluated using cell proliferation assay, nitrate assay and TNFα estimation usingELISA.
Results
Asiaticoside A inhibited the production of nitric oxide and TNFα in LPS activated primary microglia and astrocyte cultures.
Conclusion
This study suggests that Asiaticoside A may be effective against the progression of MS.
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AbbreviationsMS
Multiple Sclerosis
CNS
Central Nervous System
LPS
Lipopolysaccharide
TNFα
Tumor Necrosis Factor α
PDL
Poly D LysineKeywordsAsiaticoside
Microglia
Astrocyte
Multiple Sclerosis
Primary cell culture
… inflammatory disorders. 2. Materials and methods.
Asiaticoside A-98% (Chengdu
Biopurify Phytochemicals Ltd, China), Lipopolysaccharide (Sigma Aldrich, St. Louis,MO), Poly D Lysine Hydrobromide (Sigma Aldrich, St. Louis …
