A bioconversion technique using microorganisms has been applied to ginseng to increase content of bioactive ginsenoside and biofunctionality such as anticancer, anti-obesity and antioxidant activities. The objective of this study was to screen lactic acid bacteria for bioconversion of ginsenosides and to evaluate anti-inflammatory and cytotoxic effects of bioconverted ginseng extract. Strains isolated from kimchi were screened for their β-glucosidase activities using esculin agar. Selected strain was identified based on 16S rRNA sequencing and carbohydrate fermentation. During ginseng fermentation, viable cell number and pH were determined. Bioconverted ginsenosides were analysed by HPLC. Anti-inflammatory effects were evaluated using RAW 264.7 cells, and cytotoxic effects were determined by MTT assay. Among 166 isolates screened, Leuconostoc mesenteroides was selected for ginseng bioconversion, as it showed a higher β-glucosidase activity and viable cell number than any of the other tested strains. After fermentation for 2 days, viable cell number was 8.8 log CFU mL?1 and final pH was 4.8. Ginsenoside Rb2 was bioconverted into ginsenoside Rg3 (Rb2 → Rd → Rg3) by L. mesenteroides. The nitric oxide contents of 2-day-fermented extract decreased by as much as 25%, compared to a non-fermented extract. The cell viabilities of HepG2, HT-29, HeLa and LoVo treated with fermented ginseng extract also decreased by 49.7%, 20.2%, 21.0% and 8.7%, respectively, compared to those of control cells treated with non-fermented extract. Ginseng extract bioconverted by L. mesenteroides showed anti-inflammatory and anticancer effects. Therefore, bioconverted ginseng extract might have applications in the pharmaceutical and/or functional food industry.
… Ginsenoside standard was purchased from Chengdu Biopurify Phytochemicals Ltd. (Sichuan, China) … Ginsenoside standards (Ginsenoside Rb1, Ginsenoside Rb2, Ginsenoside Rb3, Ginsenoside Rd, Ginsenoside Rg3, Ginsenoside Rh2 and Ginsenoside CK) were purchased from Biopurify Phytochemicals Ltd. (Chengdu, China) …
