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Spirodela polyrhiza (L.) Sch. ethanolic extract inhibits LPS-induced inflammation in RAW264.7 cells

Journal name：IMMUNOPHARM IMMUNOT
Literature No.：
Literature Url： http://www.tandfonline.com/doi/abs/10.3109/08923973.2012.656273
Date publication：06 Feb 2012

Chang-Seob Seo, Mee-Young Lee, In-Sik Shin,Jin-Ah Lee, Hyekyung Ha & Hyeun-Kyoo Shin

Abstract

Objective: Spirodela polyrhiza (L.) Sch. is widely used in Korean traditional medicine. No previous work has investigated in detail the anti-inflammatory activities of S. polyrhiza or assessed in vitro their potential underlying mechanism(s). We assessed the effects of S. polyrhiza ethanolic extract (SPEE) on the production of inflammatory mediators in lipopolysaccharide (LPS)-stimulated RAW264.7 cells and investigated some potential underlying mechanisms. Additionally, we performed simultaneous determination of seven flavonoids in S. polyrhiza by high-performance liquid chromatography (HPLC)-photodiode array (PDA).

Materials and methods: RAW264.7 cells were subjected to 5, 10, 20, and 50 μg/mL of SPEE for 1 h then treated with LPS for 24 h. Production of namely nitric oxide (NO), prostaglandin E2 and cytokine levels were measured by the Griess reagent and ELISA, respectively. To investigate the underlying mechanisms of the anti-inflammatory activities of SPEE, expression of NO synthase (iNOS), cyclooxygenase-2 (COX-2), and nuclear factor-kappa B (NF-κB) proteins were evaluated by western blot analysis. HPLC analysis was performed using a Gemini C18 column at 40°C and PDA detection at 340 nm.

Orientin, vitexin, apigenin, apigetrin,luteolin, and luteoloside were purchased from Chengdu Biopurify Phytochemicals Ltd. (Chengdu, China).