HPLC-LTQ-orbitrap MSn profiling method to comprehensively characterize multiple chemical constituents in xiao-er-qing-jie granules

Yun Li,Ying Liu,Rongrong Liu,Siyi Liu,Xiuping Zhang,Zijian Wang,Jiayu Zhang and Jianqiu Lu 

Abstract

In the present study, a method based on high performance liquid chromatography coupled with linear ion trap-orbitrap mass spectrometry (HPLC-LTQ-orbitrap) was developed for a comprehensive study of the multiple chemical constituents in xiao-er-qing-jie (XEQJ) granules, which are regularly used as a traditional Chinese medicine (TCM) for the treatment of children with high fever, sore throat, and lusterless complexion. Seven major categories of constituents preliminarily isolated from the component herbs were rapidly characterized using HPLC-LTQ-orbitrap. The fragmentation patterns of these compounds with different skeletons were clearly elaborated in the electrospray ionization (ESI) collision induced dissociation (CID)-MS/MS experiments. Based on the accurate mass measurement (<5 ppm), MS/MS fragmentation patterns, diagnostic product ions, and different chromatographic behaviors, 91 compounds were unambiguously identified or tentatively characterized, including 33 phenylethanoid 

kaempferol 3-O-rutinoside, isochlorogenic acid B (3,4-DiCQA), isochlorogenic acid A (3,5-DiCQA),isochlorogenic acid C (4,5-DiCQA), tricin-7-O-glucoside, diosmetin-7-O-glucoside, phillyrin, and macranthoidin A were purchased from Chengdu Biopurify Phytochemicals CO ...