Orientin in Trollius chinensis Bunge inhibits proliferation of HeLa human cervical carcinoma cells by induction of apoptosis

Abstract

Trollius chinensis Bunge is widely used in China. Total flavonoids of Trollius chinensis Bunge contain large amounts of orientin. To ascertain whether this major fraction has a pronounced anticancer effect, the cytotoxic effect of orientin and its effect on apoptosis of HeLa cells were evaluated. The work showed orientin inhibited proliferation of HeLa cells in a dose-dependent manner. Cell growth arrest was confirmed by flow cytometry. Apoptotic signaling induced by orientin was characterized by an increased Bax/Bcl-2 ratio and up-regulation of Bax and down-regulation of Bcl-2. Orientin treatment also initiated proteolytic activation of caspase-3 and caspase-9. These results indicate that orientin may have applications in the treatment of human cervical carcinoma, and further investigation is

Orientin (98 %) was obtained from Chengdu Biopurify Phytochemicals.