Product name: Ginsenoside RK1
Synonym name: Ginsenoside RK1:Ginsenoside Rz1=5:2
Catalogue No.: BP1474
Cas No.: 494753-69-4
Formula: C₄₂H₇₀O₁₂
Mol Weight: 767.01
Botanical Source: Ginseng Radix Et Rhizoma
Physical Description:
Type of Compound: Triterpenoids

Purity: 95%~99%
Analysis Method: HPLC-DAD or/and HPLC-ELSD
Identification Method: Mass, NMR
Packing: Brown vial or HDPE plastic bottle

Storage: Store in a well closed container, protected from air and light. Put into refrigerate or freeze for long term storage.

The product could be supplied from milligrams to grams
Inquire for bulk scale.

Description:

Ginsenoside Rk1, one of the main elements of Sung Ginseng, has been confirmed as a new endothelial barrier enhancer recently and has anti-cancer activity, the mechanism involves coordination between inhibition of telomerase activity and induction of apoptosis.

References:

Arch Pharm Res. 2012 Mar;35(4):717-22.    

Induction of apoptosis by ginsenoside Rk1 in SK-MEL-2-human melanoma.    

Ginsenosides are active compounds isolated from Panax ginseng Meyer. Among these ginsenosides, less polar ginsenosides such as ginsenoside Rg3 and ginsenoside Rh2 have been demonstrated to have tumor inhibitory effects because of their cytotoxicity. 

METHODS AND RESULTS:

In this study, we evaluated the apoptotic effects of Ginsenoside Rk1 in SK-MEL-2 human melanoma. Ginsenoside Rk1 isolated from red ginseng is one of the novel ginsenosides that shows strong cytotoxicity compared to ginsenoside Rg3 in dose- and time-dependent manners. The results of DNA fragmentation, 4',6-diamidino-2-phenylindole staining, and flow cytometric analysis are corroborated that Ginsenoside Rk1 induced apoptosis in SK-MEL-2 cells. Western blot analysis revealed up-regulation of Fas, FasL, and Bax protein expression and down-regulation of procaspase-8, procaspase-3, mutant p53 and Bcl-2 protein expression. 

CONCLUSIONS:

These findings suggest that Ginsenoside Rk1 might be a promising compound to induce apoptosis through both extrinsic and intrinsic pathways in SK-MEL-2 cells.    

Biol Pharm Bull. 2008 May;31(5):826-30.    

Anti-tumor activity of the ginsenoside Rk1 in human hepatocellular carcinoma cells through inhibition of telomerase activity and induction of apoptosis.    

The Ginsenoside Rk1 is one of major components of heat-processed Panax ginseng C. A. MEYER, Sun Ginseng (SG). 

METHODS AND RESULTS:

Here, we investigated the mechanisms underlying the anti-tumor activity of Ginsenoside Rk1 in human hepatocellular carcinoma HepG2 cells in vitro. Rk1 markedly inhibited telomerase activity and cell growth along with significant morphological change. The expression levels of telomerase reverse transcriptase (hTERT) and c-Myc mRNA were obviously decreased with Ginsenoside Rk1treatment, while that of telomerase RNA (hTR) was not. Furthermore, Ginsenoside Rk1 induced apoptosis through activation of caspases-8 and -3. However, Fas-associated death domain (FADD) expression decreased with Ginsenoside Rk1 treatment, though it was known that the signaling cascade of FADD was associated with caspase-8 activity. Interestingly, activation of extracellular-regulated kinase (ERK) increased with Ginsenoside Rk1 treatment.

CONCLUSIONS:

In conclusion, these results represent the first identification of the biological activity of Ginsenoside Rk1 against HepG2 cell growth and show that the mechanism underlying the anti-tumor activity of Ginsenoside Rk1 involves coordination between inhibition of telomerase activity and induction of apoptosis.    

HPLC of Ginsenoside RK1